Investigate the effectiveness of 15 PrEST monoclonal antibodies in detecting Cancer Biomarkers with Epidermal Growth Factor Receptor Proteins

Chitra Akhila Achanta

Abstract


Western blotting is a widely used scientific method used for the analyses of specific target proteins in complex samples (13). Epidemiologic studies have shown a decrease in the death and suffering with early detection and treatment of cancer (15).  Since its emergence, cancer biomarkers have become a leading site of investigation in cancer research due to their profound ability of being able to detect cancer at all stages (20). Molecular imaging strategies are used to assess cancer biomarkers for their reliability.  Western blotting is used for both quantitative and qualitative analysis of proteins (17).  Qualitative analysis can identify the presence of specific proteins and make estimations of their abundance in a complex sample of proteins. Past research has shown a superiority of PrEST monoclonal mouse antibodies over normal antibodies in proteomic research (18).  The following study uses western blotting techniques to investigate the use of PrEST monoclonal mouse antibodies as a potential cancer biomarker.  The focus protein of the study was Epidermal Growth Factor Receptor (EGFR), a protein-coding gene, the EGFR is a member of the ErbB group of receptors associated with some physiological processes of cancer (3). The western blotting assay displayed a positive protein-protein interaction between EGFR and the cancer cells. Western blotting is an effective technique however it is not the only methods applied to determine specific protein-to protein interaction. Future research could expand testing to using other methods such as crosslinking on PrEST antibodies.


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