29 YEARS ON, A NOBEL PRIZE, AND A DIAGNOSTIC DREAM: THE USES OF THE POLYMERASE CHAIN REACTION IN MODERN BIOLOGY

Jason Seewoodhary

Abstract


This review will critically consider the uses of the polymerase chain reaction (PCR) in modern biology since its pioneering advent in 1983. PCR is an indispensible tool for use in: DNA cloning for sequencing; DNA-based phylogeny; forensic applications; and diagnostic clinical medicine, which involves diagnosing hereditary diseases, tumours and infectious diseases. The process of PCR will be outlined followed by a discussion on these uses, which will consider the limitations of PCR and how these have been overcome by the development of variations in standard PCR protocols.


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